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- Ince its publication in 1988, Antibodies: A Laboratory Manual, by Harlow and Lane, has become a classic, an essential resource for molecular biology, immunology, and cell culture labs. In order to keep the book in print, Cold Spring Harbor Laboratory Press eventually produced the paperback edition currently available for sale.
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Phage Display
Tumor antigens or cells and antibodies that attack self. An immune response may be conve-niently divided into two parts: (1) a specifi c response to a given antigen and (2) a more nonspecifi c augmentation to that response. An important feature of the specific response is that there is a quicker response to the antigen during a second exposure to.
Thank you for your interest in pComb 3, the World’s First Antibody Fab Fragments Displayed on Phage. If you would like to receive training in phage display of antibody and peptide libraries, a Cold Spring Harbor Laboratory course on the subject occurs every fall. The deadline for applications is typically July and the course is given in November. For more information, see the Cold Spring Harbor web site at www.cshl.edu.
Carlos F. Barbas, III, Laboratory Achievements:
-Development of the first phage display systems for the display of large proteins, particularly 50 KD heterodimeric antibody Fab fragments
-Development of the first phage displayed Human Antibody Libraries
-Cloning of the first High Affinity Human Monoclonal Antibodies using Phage Display
-Development of Methodologies for the construction and selection of phage display libraries of Human, Mouse, Rabbit and Chicken antibody Fab’s and scFv’s
-Development of the first Synthetic Antibody Libraries
-Development of the first Zinc Finger Display Libraries
-Development of the first Motif Grafted Antibodies
-Development of one the most potent HIV-1 neutralizing antibodies known, IgG-b12
-Development of robust methods to improve Antibody affinity- CDR Walking
-Development of the first in vitro evolved Antibodies with enhanced biological activity, the anti-HIV-1 antibody 3B3
-Development of phage-based methods for the humanization of mouse and rabbit Antibodies
-Development of a wide range of anti-Cancer Antibodies
-Development of novel Catalytic Antibodies and Catalytic peptides
Vectors designed in the Barbas lab for phage display and construction of antibody, protein, and peptide libraries including the pComb 3 vectors, related vectors for phage display and protein expression are available through Addgene. There is a fee for processing and shipping of these plasmids. See links below for descriptions and downloadable maps and sequences.
Processing of all requests are through the TSRI Office of Technology Development. Inquiries by for-profit institutes/companies can be sent to mta@scripps.edu.
Details about our plasmids and protocols can be found in:
Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, 736 pages. Information about the manual can be found by clicking on the title or by purchasing directly from www.cshlpress.com or www.Amazon.com.
1. pComb3X Set – newest version of pComb3 (pComb3XSS, pComb3XTT, pComb3XLambda)
2. pComb3H Set – 2nd generation of pComb3 (pComb3HSS, pComb3HTT, pAraH6HATT)
3. The original pCombs (pComb3, pComb8)
4. Miscellaneous (pJF3H, pIgG)
pComb3X Family (Genbank AF268281) Maps
pComb3XSS Text
pComb3XTT Text
pComb3XLambda Text
pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of SfiI cassette for cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is the vector we normally ship. The “SS” refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffer plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can also by cloned. pComb3XTT and pComb3XLambda are only needed for the construction of chimeric Fab libraries as described in Phage Display: A Laboratory Manual. The “TT” refers to the human Fab to tetanus toxin from which the kappa light chain constant region and Fd can be amplified. pComb3XLambda contains a non-functional Fab from which the lambda light chain constant region can be amplified.
Select References:
Andris-Widhopf, J.; Rader, C.; Steinberger, P.; Fuller, R.; Barbas III, C.F. (2000) Methods for the Preparation of Chicken Monoclonal Antibody Fragments by Phage Display. J. Immun. Meth., 242, 159-181.
Rader, C; Popkov, M.; Neves, J.A.; Barbas III, C.F. (2002) Integrin _v_3 Targeted Therapy of Kaposi’s Sarcoma with an In Vitro Evolved Antibody. FASEB, 16(14):2000-2.
Berry, J.D.; Rutherford, J.; Silverman, G.J.; Kaul, R.; Elia, M.; Gobuty, S.; Fuller, R.; Plummer, F.A.; & Barbas III, C.F. (2003) Development of Functional Human Monoclonal Single-Chain Variable Fragment Antibody Against HIV-1 From Human Cervical B cells. Hybridoma and Hybridomics, 22(2):, 97-108.
Jendreyko N, Popkov M, Beerli RR, Chung J, McGavern DB, Rader C, Barbas CF 3rd. (2003) Intra-diabodies: Bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors. J Biol Chem., 278(48):, 47812-9.
Steinberger, P.; Sutton, J.K.; Rader, C.; Elia, M.; and Barbas III, C. F. (2000) Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A Phage Display Approach for Rabbit Antibody Humanization. J. Biol. Chem., 275, 36073-36078.
Goncalves, J.; Kilva, F.; Freitas-Vieira, A.; Santa-Marta, M.; Malho, R.; Yang, X.; Gabuzda, D.; and Barbas III, C.F. (2002) Functional Neutralization of HIV-1 Vif Protein by Intracellular Immunization Inhibits Reverse Transcription and Viral Replication. J. Biol. Chem., 277(35):32036-45.
Popkov, M.; Mage, R.G.; Alexander, C.B.; Thundivalappil, S.; Barbas III, C.F.; Rader, C. (2003) Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: The impact of Kappa Allotype-correlated variation in cysteine content on antibody libraries selected by phage display. J. Mol. Biol., 325:325-335.
Chung, J.; Rader, C.; Popkov, M.; Hur, Y.-M.; Kim, H.-K.; Lee, Y.-J.; & Barbas III, C. F. (2004) Integrin _IIb_3 specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation,FASEB 18(2):361-3.
Popkov, M.; Jendreyko, N.; Gonzalez-Sapienza, G.; Mage, R.G.; Rader, C.; Barbas III, C.F. (2004) Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from animmune b9 allotype rabbit antibody library, J. Immunol. Methods, 288(1-2):149-164.
Popkov, M.; Rader, C.; Barbas III, C.F. (2004) Isolation of human prostate cancer reactive
antibodies using phage display technology, J. Immunol. Methods, 291:137-151.
Tanaka, F.; Fuller, R.; Barbas III, C.F. (2005) Development of Small Designer Aldolase Enzymes: Catalytic Activity, Folding, and Substrate Specificity. Biochemistry, 44:7583-7592.
pComb3H Family (Genebank AF268280) Maps
pComb3HSS Text
pComb3HTT Text
pAraH6HATT Text
pMalCSS (cytoplasmic expression) Text
pMalPSS (periplasmic expression) Text
pComb3H is the second generation of pComb vector. Improvements over pComb3 include increased stability and introduction of a new cloning region utilizing SfiI restriction sites for cloning of full Fab, scFv, peptide and other proteins for phage display. pComb3HSS contains the same double stuffer or “SS” as described above for pComb3XSS. pComb3HTT contains a human Fab to tetanus toxin which can be used as a control for Fab expression. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis. For expression of non-Fab proteins, subcloning to an expression vector with tags for detection may be necessary. We can provide arabinose inducible pAraH6HATT which contains the human Fab to tetanus toxin and a compatible SfiI cloning region as well as a similarly equipped Maltose- binding protein fusion vector pMal-SS (either cytoplasmic or periplasmic). Our version of the pMal vectors, originally obtained from New England Biolabs, are modified with the SfiI cloning cassette and SS double stuffer for easy transfer from pComb3H.
Select References:
Cary, S.P.; Lee, J.; Wagenknecht, R.; Silverman, G.J. (2000) Characterization of Superantigen-Induced Clonal Deletion with a Novel Clan III-Restricted Avian Monoclonal Antibody: Exploiting Evolutionary Distance to Create Antibodies Specific for a Conserved VH Region Surface. J. Immun., 164, 4730-4741.
Tanaka, F., Barbas III, C.F. (2001) Phage display selection of peptides possessing aldolase activity. J. Chem. Soc., Chem. Commun. 8:769-770.
Rader, C.; Cheresh, D.; Barbas III, C.F. (1998) Phage display approach for rapid antibody humanization: Designed combinatorial V gene libraries. Proc. Natl. Acad. Sci. USA, 95, 8910-8915.
Steinberger, P.; Sutton, J.K.; Rader, C.; Elia, M.; and Barbas III, C. F. (2000) Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A Phage Display Approach for Rabbit Antibody Humanization, J. Biol. Chem., 275,36073-36078.
Rader, C. and Barbas III, C.F. (1997) Phage Display of Combinatorial Antibody Libraries. Current Opinion in Biotechnology 8, 503-508.
Rader, C.; Ritter, G.; Nathan, S.; Elia, M.; Gout, I.; Jungbluth, A.A.; Cohen, L.S.; Welt, S.; Old, L.J.; Barbas III, C.F. (2000) The rabbit antibody repertoire as a novel source for the generation of therapeutic human antibodies.J. Biol. Chem., 275, 13669-13676.
Segal, D.J.; Dreier, B.; Beerli, R.R..; Barbas III, C.F. (1999) Towards controlling gene expression at will: Selection and design of zinc finger domains recognizing each of the 5’ - GNN-3’ DNA target sequences. Proc. Natl. Acad. Sci, USA, 96, 2758-2763.
Wu, H., Yang, W.-P., and Barbas III, C.F. (1995) Building Zinc fingers by selection: Towards a therapeutic application. Proc. Natl. Acad. Sci. USA, 92:344-348.
Yang, W.-P., Green, K., Pinz-Sweeney, S., Briones, A.T., Burton, D.R., and Barbas III, C.F. (1995) CDR Walking Mutagenesis for the Affinity Maturation of a Potent Human anti-HIV-1 Antibody into the Picomolar Range. J. Mol. Biol. 254:392-403.
The Original pComb
pComb3 Text & Map
pComb8 Text & Map
The original pComb3 vector was designed for phage display of Fabs which are cloned in one chain at a time using SacI/XbaI restriction sites (for the light chain) and XhoI/SpeI restriction sites (for the heavy chain). This vector system has also been used for the display of a wide variety of other proteins like zinc fingers, peptides, and cDNA fragments. Phage can be produced which express Fab or other proteins or peptides of interest fused to the phage pIII protein for expression on the head of the phage. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis. pComb8 is nearly identical but contains the phage pVIII fusion protein for multi-valent expression along the sides of the phage via fusion with pVIII.
Select References:
Barbas III, C.F. (1995) Synthetic Human Antibodies. Nature Medicine 1,837-839.
Barbas III, C.F., Bjorling, E., Chiodi, F., Dunlop, N., Cababa, D., Jones, T.M., Zebedee, S.L., Persson, M.A.A., Nara, P.L., Norrby, E. and Burton, D.R. (1992) Recombinant Human Fab fragments neutralize human type 1 immunodeficiency virus in vitro. Proc. Natl. Acad. Sci. USA 89, 9339-9343
Barbas III, C.F., Kang, A.S., Lerner, R.A. and Benkovic, S.J. (1991) Assembly of combinatorial antibody libraries on phage surfaces: The gene III site. Proc. Natl. Acad. Sci. USA 88, 7978-7982
Barbas III, C.F., Crowe, J.E., Cababa, D., Jones, T.M., Zebedee, S.L., Murphy, B.R., Chanock, R.M. and Burton, D.R. (1992) Human Monoclonal Fab Fragments derived from a combinatorial library bind to respiratory syncytial virus F glycoprotein and neutralize infectivity. Proc. Natl. Acad. Sci. USA 89, 10164-10168
Barbas III, C.F., Persson, M.A.A., Koenig, S., Chanock, R.M., Burton, D.R. and Lerner, R.A. (1992) A large array of human monoclonal antibodies to HIV-1 from combinatorial libraries of an asymptomatic seropositive individual. Vaccines '92: Modern Approaches to New Vaccines including Prevention of AIDS, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York , 9-12
Barbas III, C.F., Bain, J.D., Hoekstra, D.M. and Lerner, R.A. (1992) Semi-synthetic combinatorial antibody libraries: A chemical solution to the diversity problem. Proc. Natl. Acad. Sci. USA, 89:4457-4461.
Lerner, R.A., Kang, A.S., Bain, J.D., Burton, D.R., Barbas III, C.F. (1992) Antibodies without immunization. Science 258:1313-1314.
Barbas III, C.F., Amberg, W., Somincsits, A., Jones, T.M. and Lerner, R.A. (1993) Selection of human anti-hapten antibodies from semisynthetic libraries. Gene, 137:57-62.
Burton, D.R., Barbas III, C.F., Persson, M.A.A., Koenig, S., Chanock, R.M. and Lerner, R.A. (1991) A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Natl. Acad. Sci. USA 88, 10134-10137
Barbas III, C.F., Languino, L.R. and Smith, J.W. (1993) High Affinity Self-Reactive Human Antibodies by Design and Selection: Targeting the Integrin Ligand Binding Site, Proc. Natl. Acad. Sci. USA 90:10003-10007.
Smith, J.W., Hu, D., Satterthwait, A.C., Pinz-Sweeney, S. and Barbas III, C.F. (1994) Building Synthetic Antibodies as Adhesive Ligands for Integrins. J. Biol. Chem. 269:32788-32795.
Barbas III, C.F., and Wagner, J. (1995) Synthetic Human Antibodies: Selecting and Evolving Functional Proteins. Methods, A Companion to Methods in Enzymology 8, 94-103.
Other Related Vectors
pJF3H Text & Map
Modified version of pComb3H containing Jun, Fos and a cloning site for cDNA expression. Developed in the lab of Gregg Silverman at UCSD.
References:
Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual;
Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, , 736 pages.
pIgG Text & Map
Vector for conversion of Fab to IgG for expression in mammalian cells.
References:
Rader, C; Popkov, M.; Neves, J.A.; Barbas III, C.F. (2002) Integrin avb3 Targeted Therapy of Kaposi’s Sarcoma with an In Vitro Evolved Antibody . FASEB, 16(14), 2000-2002.
Now Available at www.cshlpress.com* or www.Amazon.com:
Phage Display: A Laboratory Manual
By Carlos F. Barbas III, The Scripps Research Institute;
Dennis R. Burton, The Scripps Research Institute;
Jamie K. Scott, Simon Fraser University;
Gregg J. Silverman, University of California, San Diego
2001, 736 pp., illus., index
Paperback $75 ISBN 0-87969-546-3
*Customers in Europe, the Middle East, and Africa should check for this publication at the CSHL Press Europe website.
Phage-display technology has begun to make critical contributions to the study of molecular recognition.
DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA.
Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage
is amplified by infection of bacteria.
Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive
instruction in its theoretical and applied so that any scientist with even modest molecular biology experience
can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying
technical expertise andexperience who attended a course on the technology at Cold Spring Harbor
Laboratory.
Phage-display technology is growing in importance and power. This manual is an unrivalled source of
expertise in its execution and application.
CONTENTS:
SECTION 1 – PHAGE DISPLAY
Chapter 1. Filamentous Phage Biology
Chapter 2. Phage-display Vectors
Chapter 3. Antibody Libraries
Chapter 4. Peptide Libraries
Chapter 5. Functional Domains and Scaffolds
Chapter 6. Gene Fragment Libraries and Genomic and cDNA Expression Cloning
SECTION 2 – ANTIBODY LIBRARIES
Chapter 7. Overview: Amplification of Antibody Genes
Chapter 8. Generation of Antibody Libraries: Immunization, RNA Preparation, and cDNA Synthesis
Chapter 9. Generation of Antibody Libraries: PCR Amplification and Assembly of Light- And
Heavy-chain Coding Sequences
Chapter 10. Selection from Antibody Libraries
Chapter 11. Analysis of Selected Antibodies
Chapter 12. Production and Purification of Fab and scFv
Chapter 13. Antibody Engineering
SECTION 3 – PEPTIDE LIBRARIES
Chapter 14. Overview: Peptide Libraries
Chapter 15. General Phage Methods
Chapter 16. Production of Peptide Libraries
Chapter 17. Screening Peptide Libraries
Chapter 18. Analysis of Phage-borne Peptides
Chapter 19. Construction and Use of pIII-displayed Peptide Libraries
SECTION 4 – OTHER LIBRARIES AND OTHER METHODS OF PANNING
Chapter 20. Construction and Selection from Gene Fragment Phage-display Expression Libraries
Chapter 21. Construction and Selection from cDNA Phage-display Expression Libraries
Chapter 22. In Vivo Selection of Phage-display Libraries
Chapter 23. Cell-surface Selection and Analysis of Monoclonal Antibodies from Phage Libraries
SECTION 5 – APPENDICES
Appendix 1. Useful Information
Appendix 2. Recipes
Appendix 3. General Procedures
Appendix 4. Cautions
Appendix 5. Suppliers
Appendix 6. Trademarks
Description
It has been 25 years since the now classic lab manual Antibodies, by Harlow and Lane appeared. This second edition ofAntibodies A Laboratory Manual Pdf
Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include:Free Antibodies Test Near Me
- 001dantibody purification and storage
- 001dengineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis
- 001dextensive labeling techniques
- 001dnew immunoblotting protocols
- 001dthe latest screening and labeling techniques
What's New in This Edition?
Antibodies A Laboratory Manual Free Download Igg
I have the first edition. Why should I buy the second edition?
While the core of the first edition appears in updated and recast form in the second edition, more than half of the second edition contains entirely new information and protocols to reflect the changes in the field since the first edition.
To whom is this edition addressed? Is this a book for immunologists?
As with the first edition, the second edition provides information and protocols for both the neophyte and the experienced investigator. It dissects the mysteries of producing the antibodies and reagents you need for your experiments with the needs of molecular biologists in mind. It is the perfect book for new members of your lab as it has sufficient background information to provide context as well as clear, step-by-step instructions for performing the needed experiments—both the why and the how for these techniques. And if things go wrong, there is extensive troubleshooting to diagnose the problem.
Are the protocols in this book better than those available for download on the Web?
Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have.